Title : 1 Intra - Uterine Calorie Restriction Affects Placental DNA Methylation and Gene 2 Expression 3 4

Title: 1 Intra-Uterine Calorie Restriction Affects Placental DNA Methylation and Gene 2 Expression 3 4 Pao-Yang Chen1, Amit Ganguly2, Liudmilla Rubbi3, Luz D. Orozco3, Marco Morselli3, 5 Davin Ashraf3, Artur Jaroszewicz3, Suhua Feng3, Steve E. Jacobsen3,4, Atsushi 6 Nakano3,5,6,7, Sherin U. Devaskar2 & Matteo Pellegrini3,5,6,8* 7 Affiliations 8 1. Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan 9 2. Department of Pediatrics, Division of Neonatology and Developmental Biology, 10 Neonatal Research Center, David Geffen School of Medicine, University of 11 California Los Angeles, Los Angeles, California 90095, USA 12 3. Department of Molecular, Cell, and Developmental Biology, University of 13 California, Los Angeles, California 90095 USA 14 4. Howard Hughes Medical Institute, University of California, Los Angeles, Los 15 Angeles, 16 CA 90095, USA 17 5. Broad Center of Regenerative Medicine and Stem Cell Research, University of 18 California, Los Angeles, Los Angeles, California 90095, USA 19 6. Molecular Biology Institute, University of California, Los Angeles, California 90095 20 USA 21 7. Jonsson Comprehensive Cancer Center, Los Angeles, California 90095 USA 22 8. Institute of Genomics and Proteomics, University of California, Los Angeles, 23 California 90095 USA 24 25 *Corresponding author 26 Corresponding author: 27 Matteo Pellegrini 28 [email protected] 29 Office Phone: (310) 825-0012 30 FAX: (310) 206-3987 31 32 33